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Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves

Received: 14 November 2014     Accepted: 19 November 2014     Published: 23 June 2015
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Abstract

The present study was conducted to investigate possible phytochemicals, cytotoxic activity, total phenolic content and antioxidant property of different extracts of Litsea glutinosa leaves. To determine different pytochemicals, various standard group tests were done. For cytotoxicity test, brine shrimp lethality bioassay was carried out. To evaluate the antioxidant properties, some complementary test systems, namely DPPH free-radical scavenging assay and reducing power assay and determination of total phenolic content were conducted. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, flavonoid, saponin, gum, steroid, tannins and terpinoids. In brine shrimp lethality bioassay, results showed that all the extracts possess significant (P˂0.05) activity when compared to the standard, Colchicine. Among the extracts, the n-hexane soluble fraction showed the highest activity (LC50 30.32±0.46µg/ml) which is very close to the standard (LC50 30.11±0.30µg/ml) used. In DPPH free radical scavenging test, IC50 value of the ethyl acetate extract was found fairly significant (9.68±0.15μg/ml) while compared to that of the reference standards ascorbic acid (1.82±0.15μg/ml). In reducing power assay, the maximum reducing capacity for the methanolic extract was found 257.67±4.04 at 200μg/ml while compared to standard ascorbic acid (356.33±5.68μg/ml). The total phenolic amount was also calculated as quite high in the ethyl acetate extract (69.00±0.58mg/g of gallic acid equivalent). Presence of significant antioxidant properties of different extracts would justify its traditional use. However, it would be interesting to investigate possible causes and their mechanisms responsible for the cytotoxic and antioxidant properties of the plant L. glutinosa.

Published in Journal of Plant Sciences (Volume 2, Issue 6-1)

This article belongs to the Special Issue Pharmacological and Biological Investigation of Medicinal Plants

DOI 10.11648/j.jps.s.2014020601.15
Page(s) 24-29
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Antioxidant Activities, Cytotoxicity, DPPH Free Radical Scavenging, Litsea Glutinosa, Total Phenolic Content

References
[1] A. Ghani, “Medicinal plants of Bangladesh: Chemical constituents and uses”, Nimtali, Dhaka: Asiatic Society of Bangladesh, 2003, pp. 285.
[2] K. Kirtikar, and B. Basu, “Indian Medicinal Plants (revised issue)”, vol.3, Allahabad, 1981, pp. 2156-2161.
[3] U. Chomkamon, S. Nongluksna, De-E. Wanchai, and R. Nijsiri, “Chemical composition and antimicrobial activity of essential oils from plants in Litsea spp.”, Technical information services (TIS)/MUTT, 2000, pp. 18-20,
[4] A. Chatterjee, and S. Pakrashi, “The Treatise of Indian Medicinal Plants”, vol. I, New Delhi, India: INSA, 1994, pp. 107.
[5] J. Loliger, “Natural antioxidants”, Lipid. Technol., vol. 3, pp. 58-61, 1991.
[6] C. Van Wagenen, R. Larsen, J. H. Cardellina, D. Ran dazzo, Z. C. Lidert, and C. Swithenbank, “Ulosantoin, a potent insecticide from the sponge Ulosa ruetzleri”, J. Org. Chem., vol. 58, pp. 335-337, 1993.
[7] S. M. R. Dewan, and A. Das, “Investigation of in vitro thrombolytic potential and phytochemical nature of Crinum latifolium L. leaves growing in coastal region of Bangladesh”, Int. J. Bio. Pharm. Res., vol. 4, pp. 1-7, 2013.
[8] T.S. Roopashree, R. Dang, S. R. H. Rani, and C. Narendra, “Antibacterial activity of anti-psoriatic herbs: Cassiatora, Momordica charantia and Calendula officinalis”, Int. J. App. Res. Nat. Prod., vol. 1, pp. 20-28, 2008.
[9] N. Meyer, N. R. Ferrighi, J. E. Putnam, L. B. Jacobsen, D. E. Nichols, J. L. McLaughlin, “Brine shrimp: A convenient general bioassay for active plant constituents”, Planta. Medica., vol. 45, pp. 31-34, 1982.
[10] S. T. Chang, J. H. Wu, S. Y. Wang, P. L. Kang, N. S. Yang, and L. F. Shyur, “Antioxidant activity of extracts from Acacia confusa bark and heartwood”, J. Agric. Food. Chem., vol. 49, pp. 3420–3424, 2001.
[11] A. Dehpour, M. A. Ebrahimzadeh, S. F. Nabavi, and S. M. Nabavi, “Antioxidant activity of methanol extract of Ferula assafoetida and its essential oil composition”, Grasas. Aceites., vol. 60, pp. 405-412, 2009.
[12] K. Wolfe, X. Wu, and R. H. Liu, “Antioxidant activity of apple peels”, J. Agric. Food. Chem., vol. 51, pp. 609-614, 2003.
[13] M. O. Ullah, M. Haque, K. F. Urmi, A. H. M. Zulfiker, E. S. Anita, M. Begum, and K. Hamid, “Anti-bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh”, Asian. Pac. J. Trop. Biomed., vol. 3, pp. 1-7, 2013.
[14] P. H. Parikh, and A. Y. Rangrez, “Extraction and Phytochemical Evaluation of Litsea Glutinosa Bark Methanolic Extract”, J. App. Pharm. Sci., vol. 2, pp. 71-78, 2012.
[15] N. Benariba, R. Djaziri, W. Bellakhdar, N. Belkacem, M. Kadiata, W. J. Malaisse, and A. Sener, “Phytochemical screening and free radical scavenging activity of Citrullus colocynthis seeds extracts”, Asian. Pac. J. Trop. Biomed., vol. 3, pp. 35-40, 2013.
[16] P. Tiwari, B. Kumar, M. Kaur, G. Kaur, and H. Kaur, “Phytochemical screening and extraction”, Int. Pharm. Sciencia., vol. 1, pp. 98-106, 2011.
[17] M. Firdaus, A. A. Prihanto, and R. Nurdiani, “Antioxidant and cytotoxic activity of Acanthus ilicifolius flower”, Asian. Pac. J. Trop. Biomed., vol. 3, pp. 17-21, 2013.
[18] S. Man, W. Gao, Y. Zhang, L. Huang, and C. Liu, “Chemical study and medical application of saponins as anti-cancer agents”, Fitoterapia., vol. 81, pp. 703-714, 2010.
[19] M. Imai, H. Kikuchi, T. Denda, K. Ohyama, C. Hirobe, and H. Toyoda, “Cytotoxic effects of flavonoids against a human colon cancer derived cell line, COLO 201: A potential natural anti-cancer substance”, Cancer. Letters., vol. 276, pp. 74-80, 2009.
[20] A. Gonzalez-Sarrias, L. Li, and N. P. Seeram, “Anticancer effects of maple syrup phenolics and extracts on proliferation, apoptosis, and cell cycle arrest of human colon cells”, J. Functional. Foods., vol. 4, pp. 185-196, 2012.
[21] S. Muruhan, S. Selvaraj, and P. K. Viswanathan, “In vitro antioxidant activities of Solanum surattense leaf extract”, Asian. Pac. J. Trop. Biomed., vol. 3, pp. 28-34, 2013.
[22] M. Elmastas, O. Isildak, I. Turkekul, and N. Temur, “Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms”, J. Food. Comp. Anal., vol. 20, pp. 337-345, 2007.
[23] A. Rezaeizadeh, Z. B. A. B. Zakaria, M. Abdollahi, G. Y. Meng, N. M. Mustapha, M. B. Hamid, and T. A. B.T. Ibrahim, “Antioxidant and antihyperglycaemic effects of an aqueous extract from Momordica charantia fruit in a type II diabetic rat model”, J. Med. Plant. Res., vol. 5, pp. 2990-2901, 2011.
[24] M. Wani, A. Sharma, J. Deshpande, S. Mathew, and M. B. Khetmalas, “Qualitative phytochemical screening of three indigenous medicinal plants”, Res. J. Pharm. Biol. Chem. Sci., vol. 4, pp. 287-291, 2013.
[25] L. Tomsone, Z. Kruma, and R. Galoburda, “Comparison of different solvents and extraction methods for isolation of phenolic compounds from Horseradish roots (Armoracia rusticana)”, World. Academy. Sci. Eng. Technol., vol. 6, pp. 903-908, 2012.
[26] X. Duan, G. Wu, and Y. Jiang, “Evaluation of antioxidant properties of phenolics from litchi fruit in relation to pericarp browning prevention”, Molecules., vol. 12, pp. 759-771, 2007.
[27] M. Ajila, K. A. Naidu, S. G. Bhat, and U. J. S. P. Rao, “Bioactive compounds of mango peel extract”, Food. Chem., vol. 105, pp. 982-988, 2007.
[28] A. Roy, K. Khanra, A. Mishra, and N. Bhattacharyya, “General analysis and antioxidant study of traditional fermented drink Handia, its concentrate and volatiles”, Adv. Life. Sci. Appl., vol. 1, pp. 54–57, 2012.
[29] Y. C. Chung, C. T. Chang, W. W. Chao, C. F. Lin, and S. T. Chou, “Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1”, J. Agric. Food. Chem., vol. 50, pp. 2454–2458, 2002.
[30] S. R. Valvi, V. S. Rathod, D. P. Yesane, “Screening of three wild edible fruits for their antioxidant potential”, Curr. Bot., vol. 2, pp. 48-52, 2011.
[31] M. Naczk, and F. Shahidi, “Phenolic in cereals, fruit and vegetables: Occurrence, extraction and analysis”, J. Pharm. Biomed. Anal., vol. 41, pp. 1523-1542, 2006.
[32] N. Nićiforović, V. Mihailović, P. Mašković, S. Solujić, A. Stojković, and D. P. Muratspahić, “Antioxidant activity of selected plant species; potential new sources of natural antioxidants”, Food. Chem. Toxicol., vol. 48, pp. 3125-3130, 2010.
[33] R. Horax, N. Hettiarachchy, and S. Islam, “Total Phenolic contents and phenolic acid constituents in 4 varieties of bitter melons (Momordica charantia) and antioxidant activities of their extracts”, J. Food. Sci., vol. 70, pp. 275-280, 2005.
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    Rumpa Bhowmick, Md. Shahid Sarwar, Syed Masudur Rahman Dewan, Abhijit Das, Binayok Das, et al. (2015). Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves. Journal of Plant Sciences, 2(6-1), 24-29. https://doi.org/10.11648/j.jps.s.2014020601.15

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    ACS Style

    Rumpa Bhowmick; Md. Shahid Sarwar; Syed Masudur Rahman Dewan; Abhijit Das; Binayok Das, et al. Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves. J. Plant Sci. 2015, 2(6-1), 24-29. doi: 10.11648/j.jps.s.2014020601.15

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    AMA Style

    Rumpa Bhowmick, Md. Shahid Sarwar, Syed Masudur Rahman Dewan, Abhijit Das, Binayok Das, et al. Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves. J Plant Sci. 2015;2(6-1):24-29. doi: 10.11648/j.jps.s.2014020601.15

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  • @article{10.11648/j.jps.s.2014020601.15,
      author = {Rumpa Bhowmick and Md. Shahid Sarwar and Syed Masudur Rahman Dewan and Abhijit Das and Binayok Das and Md. Mohiuddin and K. M. Rahat Maruf Jitu and Mohammad Safiqul Islam},
      title = {Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves},
      journal = {Journal of Plant Sciences},
      volume = {2},
      number = {6-1},
      pages = {24-29},
      doi = {10.11648/j.jps.s.2014020601.15},
      url = {https://doi.org/10.11648/j.jps.s.2014020601.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.s.2014020601.15},
      abstract = {The present study was conducted to investigate possible phytochemicals, cytotoxic activity, total phenolic content and antioxidant property of different extracts of Litsea glutinosa leaves. To determine different pytochemicals, various standard group tests were done. For cytotoxicity test, brine shrimp lethality bioassay was carried out. To evaluate the antioxidant properties, some complementary test systems, namely DPPH free-radical scavenging assay and reducing power assay and determination of total phenolic content were conducted. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, flavonoid, saponin, gum, steroid, tannins and terpinoids. In brine shrimp lethality bioassay, results showed that all the extracts possess significant (P˂0.05) activity when compared to the standard, Colchicine. Among the extracts, the n-hexane soluble fraction showed the highest activity (LC50 30.32±0.46µg/ml) which is very close to the standard (LC50 30.11±0.30µg/ml) used. In DPPH free radical scavenging test, IC50 value of the ethyl acetate extract was found fairly significant (9.68±0.15μg/ml) while compared to that of the reference standards ascorbic acid (1.82±0.15μg/ml). In reducing power assay, the maximum reducing capacity for the methanolic extract was found 257.67±4.04 at 200μg/ml while compared to standard ascorbic acid (356.33±5.68μg/ml). The total phenolic amount was also calculated as quite high in the ethyl acetate extract (69.00±0.58mg/g of gallic acid equivalent). Presence of significant antioxidant properties of different extracts would justify its traditional use. However, it would be interesting to investigate possible causes and their mechanisms responsible for the cytotoxic and antioxidant properties of the plant L. glutinosa.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Characterization of chemical groups, and investigation of cytotoxic and antioxidant activity of Litsea glutinosa leaves
    AU  - Rumpa Bhowmick
    AU  - Md. Shahid Sarwar
    AU  - Syed Masudur Rahman Dewan
    AU  - Abhijit Das
    AU  - Binayok Das
    AU  - Md. Mohiuddin
    AU  - K. M. Rahat Maruf Jitu
    AU  - Mohammad Safiqul Islam
    Y1  - 2015/06/23
    PY  - 2015
    N1  - https://doi.org/10.11648/j.jps.s.2014020601.15
    DO  - 10.11648/j.jps.s.2014020601.15
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 24
    EP  - 29
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.s.2014020601.15
    AB  - The present study was conducted to investigate possible phytochemicals, cytotoxic activity, total phenolic content and antioxidant property of different extracts of Litsea glutinosa leaves. To determine different pytochemicals, various standard group tests were done. For cytotoxicity test, brine shrimp lethality bioassay was carried out. To evaluate the antioxidant properties, some complementary test systems, namely DPPH free-radical scavenging assay and reducing power assay and determination of total phenolic content were conducted. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, flavonoid, saponin, gum, steroid, tannins and terpinoids. In brine shrimp lethality bioassay, results showed that all the extracts possess significant (P˂0.05) activity when compared to the standard, Colchicine. Among the extracts, the n-hexane soluble fraction showed the highest activity (LC50 30.32±0.46µg/ml) which is very close to the standard (LC50 30.11±0.30µg/ml) used. In DPPH free radical scavenging test, IC50 value of the ethyl acetate extract was found fairly significant (9.68±0.15μg/ml) while compared to that of the reference standards ascorbic acid (1.82±0.15μg/ml). In reducing power assay, the maximum reducing capacity for the methanolic extract was found 257.67±4.04 at 200μg/ml while compared to standard ascorbic acid (356.33±5.68μg/ml). The total phenolic amount was also calculated as quite high in the ethyl acetate extract (69.00±0.58mg/g of gallic acid equivalent). Presence of significant antioxidant properties of different extracts would justify its traditional use. However, it would be interesting to investigate possible causes and their mechanisms responsible for the cytotoxic and antioxidant properties of the plant L. glutinosa.
    VL  - 2
    IS  - 6-1
    ER  - 

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Author Information
  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Department of Pharmacy, Southeast University, Dhaka, Bangladesh

  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Department of Oncology, Ashic Oncology and Palliative Care Center, Dhaka, Bangladesh

  • Department of Pharmacy, Southeast University, Dhaka, Bangladesh

  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

  • Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh

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